Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010025_ew9d GAACGTTCTTCTTCCCAGGGCTCCATCTCTGG(+ATATAT)GTACCTAGAGATGCCTAGGTATCATCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607010024_2w6t TGGCAAGAATTACATATTCTGTATTTTTTGTG(+ATATAT)GTACCAAATGCACTGCCTTGTACTTAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240607010023_wyll TAGCACTTGCTAAGTACAAGGCAGTGCATTTG(+ATATAT)GTACCACAAAAAATACAGAATATGTAATTCT NGG 10 8 17 8 24 0 100 5 10
    240607010021_fzc6 ATCATGCTCAGACAGCTTGCTTGAGGATGTTG(+ATATAT)GATCCCCTGGTTTCTGGGGAGGCAAGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240607010019_gkft CTCAAACAAGCTTGCCTCCCCAGAAACCAGGG(+ATATAT)GATCCAACATCCTCAAGCAAGCTGTCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607010018_anfh ACTGAGCGCTCAGCTAACTTGGTGTCTGCTAG(+ATATAT)GATCCTCTGCTGTAAGCTTTACCCTGCTTTC NGG 10 8 17 8 24 0 100 5 10
    240607010016_74zm CAAAGGAAAGCAGGGTAAAGCTTACAGCAGAG(+ATATAT)GATCCTAGCAGACACCAAGTTAGCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    240607010015_s7by CACCACGCCCGGCTGCGTCACTGCTTTCTGAG(+ATATAT)GTACCATCAAATTGTTTATTACAGTTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607010014_ng6o CATGACTGCCAACTGTAATAAACAATTTGATG(+ATATAT)GTACCTCAGAAAGCAGTGACGCAGCCGGGCG NGG 10 8 17 8 24 0 100 5 10
    240607010012_heyt AAGGATTGGTGTACATCTTACCTTGTGCTGGG(+ATATAT)GATCCCAAGTTACCACACCCAGCCTCCAGAC NGG 10 8 17 8 24 0 100 5 10