Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010109_mh7y ACATGGACCCTGGGAAAGACTGCCGACTTTGG(+ATATAT)GTACCTGGGTTTCTCTACACAATGAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240607010107_ubcx ACTGTGGGGCAAGGAAAGTGACACTGACCTTG(+ATATAT)GATCCACACTGCCTGGGGTTGTGTTAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240607010106_cnyn GTCAGCTAACTAACACAACCCCAGGCAGTGTG(+ATATAT)GATCCAAGGTCAGTGTCACTTTCCTTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240607010105_lbhr AAGAAGGGAGATAGACCTCAAACTAGTTCTTG(+ATATAT)GTACCTCACCTTCATCCCCCACCCACCCACT NGG 10 8 17 8 24 0 100 5 10
    240607010104_1slv TCGGGAGTGGGTGGGTGGGGGATGAAGGTGAG(+ATATAT)GTACCAAGAACTAGTTTGAGGTCTATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607010102_6e78 GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ATATAT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607010100_ma6s GGATGGACCATCTAGTGATTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607010059_n9sb TTAGTCATCATGGATTGTCACTCCTTGAGAAG(+ATATAT)GATCCAGAGGTGTAGATTTGTTGGGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607010057_9m0y GACATACTTCCCCCAACAAATCTACACCTCTG(+ATATAT)GATCCTTCTCAAGGAGTGACAATCCATGATG NGG 10 8 17 8 24 0 100 5 10
    240607010056_luli GTGGGATTCTCTCTATGTGTGTCTTCTACATG(+ATATAT)GATCCAGAGAGCAGTTCCTAATGGAAACGAT NGG 10 8 17 8 24 0 100 5 10