Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010138_dgce TGTTTGCCAAATGTGTCCTAGTTCCCAGACTG(+ATATAT)GATCCCAACGACACAGGCATTGGGAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240607010136_vcb8 CTTTAAGGTACAAGTGTAAAAACAAGTTCCGG(+ATATAT)GATCCAGTTTCTATCCGTGTGTGTTATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607010135_7ylr GCTTTGACCATAACACACACGGATAGAAACTG(+ATATAT)GATCCCGGAACTTGTTTTTACACTTGTACCT NGG 10 8 17 8 24 0 100 5 10
    240607010134_7zl3 CTCAGTTGTCGCGGTTTGGTTTCATCGTGATG(+ATATAT)GATCCGCTGTCGTGAGTAAGGTATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240607010132_1yz0 TTCATACAATAAATACCTTACTCACGACAGCG(+ATATAT)GATCCATCACGATGAAACCAAACCGCGACAA NGG 10 8 17 8 24 0 100 5 10
    240607010131_2eob ATGTCAGACAGCACAGAAGGGAGAAGGAGGTG(+ATATAT)GATCCCCAAACCCACCTCCAGTGATCTACTT NGG 10 8 17 8 24 0 100 5 10
    240607010129_2y7z GGAGGAAGTAGATCACTGGAGGTGGGTTTGGG(+ATATAT)GATCCACCTCCTTCTCCCTTCTGTGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607010128_8hjg GACATCAGAGGGAAACACTGAATAACTTTCTG(+ATATAT)GATCCTGATTGTTGGTCTATTCTGCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240607010127_doei TTGATGTCACTGCAGAATAGACCAACAATCAG(+ATATAT)GATCCAGAAAGTTATTCAGTGTTTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607010125_6du6 AAAAAAAAGCTTGGAAAAGTGAATTTAAAGAG(+ATATAT)GATCCAAGGTAACATTCTTGAACTAAGATGC NGG 10 8 17 8 24 0 100 5 10