Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010055_mqn5 GATACATCGTTTCCATTAGGAACTGCTCTCTG(+ATATAT)GATCCATGTAGAAGACACACATAGAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607010053_oq57 GTTGCCCTTTGATGCTGAGATCTTCCGGTATG(+ATATAT)GATCCTCCGTGAAGGCAGGCTCAGGGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607010052_lx2l TCTCATCTCACCCTGAGCCTGCCTTCACGGAG(+ATATAT)GATCCATACCGGAAGATCTCAGCATCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607010050_npqy TTTTCTGAGGGTGGGGGTGGGTACTAATTGAG(+ATATAT)GATCCTTCAAGACTGAAAATCTATTGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240607010049_63qw TTATCTAAGACAATAGATTTTCAGTCTTGAAG(+ATATAT)GATCCTCAATTAGTACCCACCCCCACCCTCA NGG 10 8 17 8 24 0 100 5 10
    240607010048_d39l CCTGCTCTGTCCTCTCACTTGATCAGAGCTGG(+ATATAT)GATCCCTGCTACTTCCCAGTGCCCAAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240607010046_jkbi AGAGGAACCCTTGGGCACTGGGAAGTAGCAGG(+ATATAT)GATCCCAGCTCTGATCAAGTGAGAGGACAGA NGG 10 8 17 8 24 0 100 5 10
    240607010045_6w3a GATGTCCAGTCTTAAGGAATGAGCAATTACTG(+ATATAT)GATCCCTTACCTATCTGTCTACTGTTGGACT NGG 10 8 17 8 24 0 100 5 10
    240607010043_0xtf CAGACAGTCCAACAGTAGACAGATAGGTAAGG(+ATATAT)GATCCAGTAATTGCTCATTCCTTAAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240607010042_bfvh CCTCTCGGGCTAGGAGGGCGCTGGATGGCGGG(+ATATAT)GTACCCTCCCTCCTGCACCGACTCCACTGAA NGG 10 8 17 8 24 0 100 5 10