Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010040_jv2f ACTACTTCAGTGGAGTCGGTGCAGGAGGGAGG(+ATATAT)GTACCCCGCCATCCAGCGCCCTCCTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240607010038_4s2g ATACACAGTCCGGTGGGGGAGGGGCTGGGCTG(+ATATAT)GATCCTGGTAGGGGTAAGCGGTAGTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607010037_vcu1 CTGCAAGCTCCACTACCGCTTACCCCTACCAG(+ATATAT)GATCCAGCCCAGCCCCTCCCCCACCGGACTG NGG 10 8 17 8 24 0 100 5 10
    240607010035_hffl AAAATAATATTTGGGTGAATGATACTGCCATG(+ATATAT)GATCCTTTTGTTTAATTTTATATATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607010034_gcd1 TATCCGAGCTTATATATAAAATTAAACAAAAG(+ATATAT)GATCCATGGCAGTATCATTCACCCAAATATT NGG 10 8 17 8 24 0 100 5 10
    240607010032_rp4n TCACAAGCAGGTGTTTATGTCTGAAATTGGAG(+ATATAT)GTACCCATTATCTGAAAATTCCTCTCCCATA NGG 10 8 17 8 24 0 100 5 10
    240607010031_9ssx TACCTTATGGGAGAGGAATTTTCAGATAATGG(+ATATAT)GTACCTCCAATTTCAGACATAAACACCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607010029_hzqp CACTTTAAAACACTGTGGAGCCAGGAATAATG(+ATATAT)GTACCCACCTGTAATCCCAGCACCTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607010028_x62b TCCTGTCTCCCAGGTGCTGGGATTACAGGTGG(+ATATAT)GTACCATTATTCCTGGCTCCACAGTGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607010027_nfyq CATGTCTCTGGATGATACCTAGGCATCTCTAG(+ATATAT)GTACCCAGAGATGGAGCCCTGGGAAGAAGAA NGG 10 8 17 8 24 0 100 5 10