Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003305_x53w CTGTTCTTGCCCCCGTCACATTCTGGAAAGGG(+ATATAT)GTACCCAGTGATCTCTGTGACGCCTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607003304_1pu0 GAGGAGTCTGGAGGCGTCACAGAGATCACTGG(+ATATAT)GTACCCCTTTCCAGAATGTGACGGGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240607003302_a2j0 TTTAAACCTTTCAAAACAAAATTCCTATCCAG(+ATATAT)GATCCCATCCAGAGTCATCACCTGCCACTCA NGG 10 8 17 8 24 0 100 5 10
    240607003300_uotp CTTGTTGAGTGGCAGGTGATGACTCTGGATGG(+ATATAT)GATCCTGGATAGGAATTTTGTTTTGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240607003259_bpkx GGGAGGCCATGCCCGCCACCCCTCTTGTTCTG(+ATATAT)GATCCCTGCTCCCCTCTGGGTGTGTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607003257_6rnu CACACACACCCACACACCCAGAGGGGAGCAGG(+ATATAT)GATCCAGAACAAGAGGGGTGGCGGGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240607003256_z31l AGGCAATTAGAAAAATTATCCAAATAAATTCG(+ATATAT)GTACCTAGGAAAAAGAAAATAATGTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607003255_tkd2 GAGATAAGGAAACATTATTTTCTTTTTCCTAG(+ATATAT)GTACCGAATTTATTTGGATAATTTTTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607003254_bzjm GTCACCAGTCACTGGGTCTAGGAGATGTCTTG(+ATATAT)GTACCCGTCTGTCTGACATTGACAATGACCT NGG 10 8 17 8 24 0 100 5 10
    240607003252_gz9r CTTTCAGGTCATTGTCAATGTCAGACAGACGG(+ATATAT)GTACCAAGACATCTCCTAGACCCAGTGACTG NGG 10 8 17 8 24 0 100 5 10