Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003118_dwkn AGGTTGTGGGTATATTCAGCAGGCTGCCTTAG(+ATATAT)GTACCATCGGGGGAGCAGACAGACAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607003116_i5p3 CCAGGAGGCCTGACCTGGCCGGAGTGGACAGG(+ATATAT)GATCCAGAAACGAAGCCAAATAAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607003114_io5r GTGGCCTCTTCCCTTATTTGGCTTCGTTTCTG(+ATATAT)GATCCCTGTCCACTCCGGCCAGGTCAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607003112_hy6d GTCTGCAACTATCCCTAACTCCAGGTCCAGGG(+ATATAT)GATCCCATGCCGTCTTCTGACTTCTGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607003110_61k9 CTTGTGCCCTCAGAAGTCAGAAGACGGCATGG(+ATATAT)GATCCCCTGGACCTGGAGTTAGGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607003109_uqfz GCACCAGCAGCATCCAATCAACCTGTACTATG(+ATATAT)GATCCTCCAACATCTAAGCAATAATGCCCTA NGG 10 8 17 8 24 0 100 5 10
    240607003107_b0u3 AGCTGTAGGGCATTATTGCTTAGATGTTGGAG(+ATATAT)GATCCATAGTACAGGTTGATTGGATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607003106_2t0y ACACATTTGTGATAAGACAAGGAAGTCTACAG(+ATATAT)GATCCTCCATCTCTTAGTCTTGCTGAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607003105_edf5 TGAAATGACCTCAGCAAGACTAAGAGATGGAG(+ATATAT)GATCCTGTAGACTTCCTTGTCTTATCACAAA NGG 10 8 17 8 24 0 100 5 10
    240607003103_sd6q GGAGGGGACCCGATTCCCGCCAAATAATCTCG(+ATATAT)GATCCAGTAGCAGGGTGAGCACGTGATGGCT NGG 10 8 17 8 24 0 100 5 10