Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003015_yh42 CAATTAAGAGCACTCCCTGCTTGCAGAGTTTG(+ATATAT)GATCCCAAATCAGGCAGGTAGAGCTGTAACT NGG 10 8 17 8 24 0 100 5 10
    240607003014_r9fb ACCACATAGTGGCTCATAACCATCTGTAATGG(+ATATAT)GATCCAATGCCATCTTCTGGTATCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607003013_o16v GCTGTCTTCAGAGATACCAGAAGATGGCATTG(+ATATAT)GATCCCATTACAGATGGTTATGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240607003011_95d7 AGTCACCGCCTGGCAAGGGCCATGGTAGTTAG(+ATATAT)GTACCGAAATGCTCCTGTACAGATGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240607003010_v06o GTACAGGTTTGCATCTGTACAGGAGCATTTCG(+ATATAT)GTACCTAACTACCATGGCCCTTGCCAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240607003008_0n8g CTAGCTGGGAGTGCCGGCAGAGGCAGGGCTGG(+ATATAT)GTACCACAGGGCCAGCTTGAGTGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607003006_cdzo CAAGTTCCTCCCACACTCAAGCTGGCCCTGTG(+ATATAT)GTACCCAGCCCTGCCTCTGCCGGCACTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607003003_3kni TCCATGCCTCCCCACCCTTGTTCACGCCCATG(+ATATAT)GATCCCCTGGCCTTGATGTCTGCCCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607002959_19ig CTGAGAAAGAGGGGCAGACATCAAGGCCAGGG(+ATATAT)GATCCATGGGCGTGAACAAGGGTGGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240607002958_piz0 CTCCCCAGAGGACGCAGTGAGTCCCCAGCTGG(+ATATAT)GATCCCACCACACTGGACAGGAGCCAGGAGT NGG 10 8 17 8 24 0 100 5 10