Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003040_7cfn ACCCAGCCCCCCATTTGATATGTAGGCAGGAG(+ATATAT)GTACCTAAGTAGGAAACAAGTCAGATTATAT NGG 10 8 17 8 24 0 100 5 10
    240607003039_ssvb TAAGCATATAATCTGACTTGTTTCCTACTTAG(+ATATAT)GTACCTCCTGCCTACATATCAAATGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607003037_nuxk TTTTCCTCTGCAGCGCCGCTCTCTGCAGCCGG(+ATATAT)GATCCACCACCCACTTTCTCACGGCCATCTC NGG 10 8 17 8 24 0 100 5 10
    240607003036_2ms1 TGGAAGAGATGGCCGTGAGAAAGTGGGTGGTG(+ATATAT)GATCCCGGCTGCAGAGAGCGGCGCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607003034_8t6q TGGGAGGAAGAGGAAGGCAGCTCCTGCTCCTG(+ATATAT)GATCCCCCTTGAAGATCCATTCTCCATAGAA NGG 10 8 17 8 24 0 100 5 10
    240607003031_g6zb TTTGCTTCTATGGAGAATGGATCTTCAAGGGG(+ATATAT)GATCCAGGAGCAGGAGCTGCCTTCCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607003029_yhft CAGACTGGGAGGGGACGGGTAAGAGAGGTGGG(+ATATAT)GTACCAGACTCCAACAGGCTCCAAAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607003028_68mi TTTTCTATTTCTTTGGAGCCTGTTGGAGTCTG(+ATATAT)GTACCCCACCTCTCTTACCCGTCCCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607003026_uoo0 GTAGGGCCAGCGGGCGCCGGAAGTGATTCCAG(+ATATAT)GTACCCTTTCCCCCACCTTGGAGTCTGACAA NGG 10 8 17 8 24 0 100 5 10
    240607003025_f4hw GAGACTTGTCAGACTCCAAGGTGGGGGAAAGG(+ATATAT)GTACCTGGAATCACTTCCGGCGCCCGCTGGC NGG 10 8 17 8 24 0 100 5 10