Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002956_c0mp AGAGCACTCCTGGCTCCTGTCCAGTGTGGTGG(+ATATAT)GATCCCAGCTGGGGACTCACTGCGTCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607002954_miji TGACTCCTTCAGTGTCTATAGAAGAAAAGGTG(+ATATAT)GATCCTGGAGCCTACAGAAAACTCAACCCTT NGG 10 8 17 8 24 0 100 5 10
    240607002953_eohx CCTAGAAGGGTTGAGTTTTCTGTAGGCTCCAG(+ATATAT)GATCCACCTTTTCTTCTATAGACACTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607002951_imh8 TGACTCCTTCAGTGTCTATAGAAGAAAAGGTG(+ATATAT)GATCCTGGAGCCTACAGAAAACTCAACCCTT NGG 10 8 17 8 24 0 100 5 10
    240607002949_wtqi CCTAGAAGGGTTGAGTTTTCTGTAGGCTCCAG(+ATATAT)GATCCACCTTTTCTTCTATAGACACTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607002947_hy6e GGGAGCCAGTGGTTTCTGAAGACTGTCCAGGG(+ATATAT)GATCCCTAGTTCAGAGTGGCCCCATCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607002945_r1jq CATGTTGCCTGATGGGGCCACTCTGAACTAGG(+ATATAT)GATCCCCTGGACAGTCTTCAGAAACCACTGG NGG 10 8 17 8 24 0 100 5 10
    240607002944_4ut9 AGGGAGGTGTGCGATGTTAGCCTTCTGCTTGG(+ATATAT)GATCCTTTGACCGGGAGGCCTGTAGTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607002942_it26 ACTTCAGGACACTACAGGCCTCCCGGTCAAAG(+ATATAT)GATCCCAAGCAGAAGGCTAACATCGCACACC NGG 10 8 17 8 24 0 100 5 10
    240607002940_sps1 GGCCCTGCTAGATGCTGACATCAGCCCCAGGG(+ATATAT)GTACCTTTCTGCTCTGCAGGACTGATCTCAT NGG 10 8 17 8 24 0 100 5 10