Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002852_3g7b GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+ATATAT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607002850_rlf1 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+ATATAT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607002848_ic5g GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+ATATAT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607002847_f5fj CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+ATATAT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607002845_wpqp GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+ATATAT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607002843_3u2q CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+ATATAT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607002842_7jfw GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+ATATAT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607002841_dsmk CATGTCTCTGCAGAGGCCAGAGGAGGGTATTG(+ATATAT)GATCCCTAGAAGCTGGAGTTAGAATAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240607002839_cayg GAGACTTACCTATTCTAACTCCAGCTTCTAGG(+ATATAT)GATCCAATACCCTCCTCTGGCCTCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607002838_qbl2 GTTCCAGCAAACAGGAAATGGGGATGCTGAAG(+ATATAT)GATCCCAGGGAGGGGACGTTACAACCACAGC NGG 10 8 17 8 24 0 100 5 10