Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002750_di9s AAGAAAACACTTTAGGATGCTGAGCTAAATTG(+ATATAT)GATCCCCTTCCTCGTTCCTCTCACCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607002749_n4j1 CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+ATATAT)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240607002747_ppex CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+ATATAT)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240607002744_gkzw GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+ATATAT)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240607002742_o3b3 ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+ATATAT)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607002741_cu1a CTATAGATGGAGATCTGAAGATTAGACTCTAG(+ATATAT)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240607002739_gywa AGGCAAATGTCACGTATACTCTCTCATCTGTG(+ATATAT)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240607002738_kpmy CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+ATATAT)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240607002736_fpdm TAATTTATGACATCTGATACTGTTTCTGGTGG(+ATATAT)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240607002735_fmnq CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+ATATAT)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10