Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002827_toft GGAGGTGACCTCAAGGACTCTGTGGGGATTTG(+ATATAT)GATCCGGCTGGGCTCCGGCTTGAGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607002825_4xsq ATTTTCCAGCCTCTCAAGCCGGAGCCCAGCCG(+ATATAT)GATCCAAATCCCCACAGAGTCCTTGAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607002824_9qd7 CGACTGAGACAGGTCAGAATCGAGCCAGCTAG(+ATATAT)GATCCGTCTGTGCCTAGGGAGGAAATGGGGT NGG 10 8 17 8 24 0 100 5 10
    240607002822_lx0o GCATTACCCCATTTCCTCCCTAGGCACAGACG(+ATATAT)GATCCTAGCTGGCTCGATTCTGACCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607002821_wd0u GGCCACAGAGAGGTGGGAAGGATTTGTTGAGG(+ATATAT)GATCCCAGGATCAAAGGAGACTGCCATGACC NGG 10 8 17 8 24 0 100 5 10
    240607002818_0ydc CCTTTGGTCATGGCAGTCTCCTTTGATCCTGG(+ATATAT)GATCCCTCAACAAATCCTTCCCACCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607002817_f2l7 ACGAAGGGCCAGCTTGAGGAAAGCCTCAGGTG(+ATATAT)GTACCAAAATTAGCTGCCGTCTGCCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607002816_ucss CACCAGCAGGGGGCAGACGGCAGCTAATTTTG(+ATATAT)GTACCACCTGAGGCTTTCCTCAAGCTGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607002814_nths TAGGGAACAGGGAAATCTTTGGTCTGGGGCTG(+ATATAT)GATCCTGGAGCTCAAGTCCAGGGAAGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607002812_6phw CCTTCCCCTGCTTCCCTGGACTTGAGCTCCAG(+ATATAT)GATCCAGCCCCAGACCAAAGATTTCCCTGTT NGG 10 8 17 8 24 0 100 5 10