Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002810_4sdq TCTCAGGAGGTCCCTTCTCAAGGACACGCGGG(+ATATAT)GTACCTCTCACCCAACTACCAACAGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607002809_zeal TGGCTTTCCTGCTGTTGGTAGTTGGGTGAGAG(+ATATAT)GTACCCCGCGTGTCCTTGAGAAGGGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240607002808_tezg AGAAGCAGGAGGGTGTGGGTTGTGGGTGACAG(+ATATAT)GTACCTCACAGGGTCTCAATTATCGCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240607002806_c7kv GCAGAGTCAAGCGATAATTGAGACCCTGTGAG(+ATATAT)GTACCTGTCACCCACAACCCACACCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607002805_2rdf GAGAGAATGTCTCTCACCCACCGGGAGTCAGG(+ATATAT)GATCCATCAACGAGTGGGGCTGGGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607002803_eiv8 TCTATTCCTGCCCCCAGCCCCACTCGTTGATG(+ATATAT)GATCCCTGACTCCCGGTGGGTGAGAGACATT NGG 10 8 17 8 24 0 100 5 10
    240607002802_rlwt GTCTGGGCTGCCAGCATTTCCCAGTGTTTCAG(+ATATAT)GATCCTGAAGGCACAGAAAACGTTGAGATCG NGG 10 8 17 8 24 0 100 5 10
    240607002801_jb78 TCCCCCGATCTCAACGTTTTCTGTGCCTTCAG(+ATATAT)GATCCTGAAACACTGGGAAATGCTGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607002800_h53v AAAAAAAAAAAAATACCGTGGGAAAACAAGTG(+ATATAT)GTACCGTAATATTCCGAGTATAGGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607002759_wsyf AATGGTTGGGGACCTATACTCGGAATATTACG(+ATATAT)GTACCACTTGTTTTCCCACGGTATTTTTTTT NGG 10 8 17 8 24 0 100 5 10