Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001517_duvq GAGTGTGAGTCAAGAGATGGGTGCTTAGAGAG(+ATATAT)GATCCAGGGTGGTGGGGTGAGTTGGAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240607001514_wngj GACTCTTGTCAAGAGGACCCGCACGAAGCCCG(+ATATAT)GATCCACAGCGAGCTTTTTGGAAGGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240607001513_2qdy AACTGTTATTCCCTTCCAAAAAGCTCGCTGTG(+ATATAT)GATCCGGGCTTCGTGCGGGTCCTCTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240607001510_dsnq GCTCACAACCATCTGTATCTCCAGTCCCAGGG(+ATATAT)GATCCAGCACCCTCTTCTGGTGTCCTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607001508_kou0 CTGGTGTCTGAGGACACCAGAAGAGGGTGCTG(+ATATAT)GATCCCCTGGGACTGGAGATACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607001507_pvkj GAAGCTTAGTGAGGCGGAGGCTGCCAGTGTTG(+ATATAT)GTACCTGTGGATTTTCCAATAGTGAGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240607001505_ouh9 AGAGCTAACCCTCACTATTGGAAAATCCACAG(+ATATAT)GTACCAACACTGGCAGCCTCCGCCTCACTAA NGG 10 8 17 8 24 0 100 5 10
    240607001503_1cip GGTCACAGTGAAGTCCCTTGCAGCCCCCAGTG(+ATATAT)GTACCTTACACCAAGGACTGTGCTCTCAATC NGG 10 8 17 8 24 0 100 5 10
    240607001502_itjc ACAGTGATTGAGAGCACAGTCCTTGGTGTAAG(+ATATAT)GTACCACTGGGGGCTGCAAGGGACTTCACTG NGG 10 8 17 8 24 0 100 5 10
    240607001500_ptnu GAAAGTTGTTTTCTTACTCTGTCCTTGGCAGG(+ATATAT)GTACCTCTTACATTTCCCAGATACGGTTTGA NGG 10 8 17 8 24 0 100 5 10