Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001543_pv56 TTGGCTAAACTGGTTGAACTACAATTCCCATG(+ATATAT)GATCCTCTTGTCTTTATCTCCCAACTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607001542_79q9 AGTGTCTGGGTTTGGTGGTTGTATATGGGATG(+ATATAT)GATCCCTGGGTGGGGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607001539_ijey GGAAAGACCATCCAGAGACTGCCCCACCCAGG(+ATATAT)GATCCATCCCATATACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607001538_ev65 TGCTAAGTTCAGGGATGGGAGAATTGCACAAG(+ATATAT)GTACCTCTTGGGCTGGGAAGTTTAGCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240607001537_uwti CATCCCAAAAGCTAAACTTCCCAGCCCAAGAG(+ATATAT)GTACCTTGTGCAATTCTCCCATCCCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240607001536_tr25 TACAGACCCAGTCTCCAGCTGTTTTGACACTG(+ATATAT)GATCCTTGTAAAACTGTACAAGTCATTTGTA NGG 10 8 17 8 24 0 100 5 10
    240607001535_qg4w TGAATTACAAATGACTTGTACAGTTTTACAAG(+ATATAT)GATCCAGTGTCAAAACAGCTGGAGACTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607001533_h5vh GCTGGAGAGCTGCTCACACAGCTGGACTTCTG(+ATATAT)GATCCTTACACTCACACCCGCTTTCATTTGG NGG 10 8 17 8 24 0 100 5 10
    240607001532_wu32 AAGTACCAAATGAAAGCGGGTGTGAGTGTAAG(+ATATAT)GATCCAGAAGTCCAGCTGTGTGAGCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607001531_pj02 TATTTTCAAATGTGTTCCTCCCTGGAAAATAG(+ATATAT)GATCCTTCTTACACAAACCTGTATTTTATAG NGG 10 8 17 8 24 0 100 5 10