Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001646_oio7 CCAGAAATGACCATGTGATAGTCTCAGGGCAG(+ATATAT)GTACCTACCCTGAGAAAGGCCTGACAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607001644_kruj CCTTTCTCAGTCACAAGCCATGACGACATGGG(+ATATAT)GATCCGGACGCTTTGTGCTCTTAGCTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607001643_9lxk CGGCCACAGAAGCTAAGAGCACAAAGCGTCCG(+ATATAT)GATCCCCATGTCGTCATGGCTTGTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240607001640_7twn GAAGCTCAGGCAGAAAGCCAACGGCTCCCAAG(+ATATAT)GATCCACCCTGAGGAGCTAAGCAACGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240607001638_a4zi TGAGCGAGCGCGTTGCTTAGCTCCTCAGGGTG(+ATATAT)GATCCTTGGGAGCCGTTGGCTTTCTGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607001637_gfa2 ATGCAACACTGGGTGTTCACCTGCCTTACTGG(+ATATAT)GATCCCATGTGTTTAGTAATTTAACGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607001635_38k9 TTCAGCTGGACGTTAAATTACTAAACACATGG(+ATATAT)GATCCCAGTAAGGCAGGTGAACACCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607001634_pijz AGACTGCTGGGAAACCCAGGCCAAAGGGCAGG(+ATATAT)GATCCAGACTGGCCATCTCATTGTACACTGT NGG 10 8 17 8 24 0 100 5 10
    240607001632_sapj ATGACACAGTGTACAATGAGATGGCCAGTCTG(+ATATAT)GATCCCTGCCCTTTGGCCTGGGTTTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607001630_80d1 GCATGGATTTGATCATGGGGGCCAGGGGCCAG(+ATATAT)GTACCCCTGACCCTCTCAGTGTGGAGAGATG NGG 10 8 17 8 24 0 100 5 10