Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001629_sv6y AGGTGCATCTCTCCACACTGAGAGGGTCAGGG(+ATATAT)GTACCTGGCCCCTGGCCCCCATGATCAAATC NGG 10 8 17 8 24 0 100 5 10
    240607001627_1wre ACTTGCTTTTTTTTTTTTTTAATTTGATGCTG(+ATATAT)GATCCAGAATAGGCCAGTGAAGATCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607001626_74dh ACCACTGCTCTGATCTTCACTGGCCTATTCTG(+ATATAT)GATCCAGCATCAAATTAAAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240607001625_p6ax TAACTTGAACTTGCAGGTCACCTCTGAACAGG(+ATATAT)GTACCCTTGTCCCCACCCCAGGCCCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607001623_xscz ACAGGGTAACAGGGCCTGGGGTGGGGACAAGG(+ATATAT)GTACCCTGTTCAGAGGTGACCTGCAAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240607001620_5g65 TCTCCTGTGTAGACTAGCTGGAGGTCCCTGGG(+ATATAT)GATCCCCAGGGAAAGGACGCTCTGCCGCGCA NGG 10 8 17 8 24 0 100 5 10
    240607001618_jti0 GAGATTGCGCGGCAGAGCGTCCTTTCCCTGGG(+ATATAT)GATCCCCAGGGACCTCCAGCTAGTCTACACA NGG 10 8 17 8 24 0 100 5 10
    240607001616_9gln CTTTGTCCTCGTCGAGCTGCTCTGAGCTGTGG(+ATATAT)GATCCTGTGAACAACTGTCGTCTTTCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607001615_zxyz TGAAGAGAAGGAAAGACGACAGTTGTTCACAG(+ATATAT)GATCCCACAGCTCAGAGCAGCTCGACGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607001613_qidp CCAAAATGCCCGTCCTATAGAGTGTCTGATTG(+ATATAT)GATCCTCTGTTGCGTATTCTGATGGTGCCTG NGG 10 8 17 8 24 0 100 5 10