Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001730_lfka AATCGTAAGGGTTGTTGAAGGCAGATACTGAG(+ATATAT)GATCCTAAGTGAAAGCCTCATGTATCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607001729_5imz TCCCCAAAATCGTTTGAATTAGATTCTGCCTG(+ATATAT)GTACCTGTGTTCTGATTGGTCCTGGAGTATA NGG 10 8 17 8 24 0 100 5 10
    240607001727_q42d CCTCGTATACTCCAGGACCAATCAGAACACAG(+ATATAT)GTACCAGGCAGAATCTAATTCAAACGATTTT NGG 10 8 17 8 24 0 100 5 10
    240607001726_9ll3 TGAAAGCATTACTATATTTTTTACATTAAGTG(+ATATAT)GTACCAATAAAGTATTTTGTTACCAAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240607001725_3qvy CATTTAGCTTTTGGTAACAAAATACTTTATTG(+ATATAT)GTACCACTTAATGTAAAAAATATAGTAATGC NGG 10 8 17 8 24 0 100 5 10
    240607001724_zhow CTTCAAAGTCACGGGCCAGCCAGACACAAAAG(+ATATAT)GTACCAGTCAAAGTCGCATTGTGGGAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240607001722_4z0l ACAGTCTAGCTCCCACAATGCGACTTTGACTG(+ATATAT)GTACCTTTTGTGTCTGGCTGGCCCGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240607001721_5hda GTTTCGGAAGTTGATTCATGTAGCTGACAGTG(+ATATAT)GATCCAGAAGGGTCAGTCCCCTTCACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607001720_e0hw ACAAGATGGAGTGAAGGGGACTGACCCTTCTG(+ATATAT)GATCCACTGTCAGCTACATGAATCAACTTCC NGG 10 8 17 8 24 0 100 5 10
    240607001718_8v5y ATAGTACCCATGGTGTGTTCTGATCGATCCTG(+ATATAT)GATCCCCGGACTCCTTGGGACATGCTATTGA NGG 10 8 17 8 24 0 100 5 10