Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001747_z8qz GATGTCTGAGACTGGAGTATTCTCTTGGCCGG(+ATATAT)GATCCACTCACCGCTGTGCGGCATAGGATGT NGG 10 8 17 8 24 0 100 5 10
    240607001745_u2cd TTAGGCAAAGTTAATAGGGTATCTGGCATTGG(+ATATAT)GATCCTACTGACTGCCATTCAGTAAGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240607001744_05qm TAACTATTGGCTTACTGAATGGCAGTCAGTAG(+ATATAT)GATCCCAATGCCAGATACCCTATTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240607001742_04ds TTCAGGGAAAAAGAAAACATGTTTTACCTAGG(+ATATAT)GATCCTTCTGAACAAAATGGCTGTCTGTACT NGG 10 8 17 8 24 0 100 5 10
    240607001740_toe9 AAAAGAGTACAGACAGCCATTTTGTTCAGAAG(+ATATAT)GATCCCTAGGTAAAACATGTTTTCTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607001738_drkj ATCAGGGGTTGGGTCCTGGACGACAACACAGG(+ATATAT)GATCCTTTGAGACCCTCTTAAGTGGGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607001737_4bwp CATACTTTGGCCCACTTAAGAGGGTCTCAAAG(+ATATAT)GATCCCTGTGTTGTCGTCCAGGACCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240607001735_x075 CAGAGAGCACCGGCTGCTGTTCAGGATCACAG(+ATATAT)GATCCTCTAGGCTGGGCCACAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607001733_re1n AAGAGGAAGGAAGACTGTGGCCCAGCCTAGAG(+ATATAT)GATCCTGTGATCCTGAACAGCAGCCGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607001732_g9ls GAAAGAAACAGATACATGAGGCTTTCACTTAG(+ATATAT)GATCCTCAGTATCTGCCTTCAACAACCCTTA NGG 10 8 17 8 24 0 100 5 10