Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001810_dcjv GGTCACTGCAGGGGAATGGTCTCAAGCTGCAG(+ATATAT)GATCCTAACCTGGAAGAGGAAGCCATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607001809_d6mc TGAACAGACCATGGCTTCCTCTTCCAGGTTAG(+ATATAT)GATCCTGCAGCTTGAGACCATTCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240607001808_c1bh ATCGATCCAGACGAGGTCAGGGCTGTGCTGTG(+ATATAT)GATCCATCACACAAGCGTCTGTCCATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607001807_x3pl GAGAGATGGGATGGACAGACGCTTGTGTGATG(+ATATAT)GATCCACAGCACAGCCCTGACCTCGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607001805_it34 AAGCCTCTGCACTCTTGCCATCCCGTGTCAGG(+ATATAT)GATCCAGTGTGGGTGGGCGGCTGAGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240607001804_poxn GGAAGGAGGTCCTCAGCCGCCCACCCACACTG(+ATATAT)GATCCCTGACACGGGATGGCAAGAGTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240607001802_l7h2 AAAACAGCAGCCACCTATGGACCAGTCCATTG(+ATATAT)GTACCATACCAATCTGGAAAGGGCACGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607001801_90ny CCCAGCTGGCGTGCCCTTTCCAGATTGGTATG(+ATATAT)GTACCAATGGACTGGTCCATAGGTGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607001759_xdb2 GGCGCAGGAGGGGCCTGCCCCCCCACACACTG(+ATATAT)GATCCCCAGTGGCCAAGTGCCCCGGGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240607001757_7csi ATGTGAAAGGCCCGGGGCACTTGGCCACTGGG(+ATATAT)GATCCAGTGTGTGGGGGGGCAGGCCCCTCCT NGG 10 8 17 8 24 0 100 5 10