Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182010_69ls ATGTATCACGCTGTTAGAAACCCAAGTCAGAG(+AGGACA)GTACCTTTATCTAGCCACGCCCTAGCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613182009_gqku AGTTTAAACCTACATACCCAGTTTCCTTTCAG(+AGGACA)GTACCTTGACCTGCTTCCAGGGGAACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613182008_crv1 GCTATTAAAAGTTCCCCTGGAAGCAGGTCAAG(+AGGACA)GTACCTGAAAGGAAACTGGGTATGTAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613182006_z4tz TTCCAAGGATGGAACTCAGGTGGTTAGTATTG(+AGGACA)GTACCAGGAACCTAAACTTACCCTTTGAACC NGG 10 8 17 8 24 0 100 5 10
    240613182004_l59f GGGTTGGTTCAAAGGGTAAGTTTAGGTTCCTG(+AGGACA)GTACCAATACTAACCACCTGAGTTCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240613182003_a6eb AATGCCCTTTCGTGCTTTGTCACCTACACTCG(+AGGACA)GTACCCAGGACCTGGTGCTATTAACTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613182000_i4yu GAAGGCACAGAGTTAATAGCACCAGGTCCTGG(+AGGACA)GTACCGAGTGTAGGTGACAAAGCACGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613181959_1onp TCAGGGAAGTATGTGTCTCCCGGGGAGGGATG(+AGGACA)GTACCTCCGTGGGCATCCCCCAAAGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613181957_hfww TGGTGGAGGGCCTTTGGGGGATGCCCACGGAG(+AGGACA)GTACCATCCCTCCCCGGGAGACACATACTTC NGG 10 8 17 8 24 0 100 5 10
    240613181956_3nwo CTCCACTTTATTTTTGAAATGCATACTTATTG(+AGGACA)GTACCCTTTTGTTTCTTCCCTCACAAAAATG NGG 10 8 17 8 24 0 100 5 10