Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181924_c2xl AAGCAGGAAGAAAGCTACAAAACTGGTATTTG(+AGGACA)GTACCATGTGTGAGCCTGGTTAAATTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613181922_cfkg GGTGTTATCTGAAGCAGGGCAGTGTGGTTTAG(+AGGACA)GATCCTTAGCACCACAAAAAACAAAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613181921_mu8g ACTAGGCTGGCTTTGTTTTTTGTGGTGCTAAG(+AGGACA)GATCCTAAACCACACTGCCCTGCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240613181920_8226 AAATTATAAAGCCCCATAAGCAAGTCACTTTG(+AGGACA)GTACCATTCCTGAGAAAGAAGTTTACATATA NGG 10 8 17 8 24 0 100 5 10
    240613181918_pdv9 TTTATTATATGTAAACTTCTTTCTCAGGAATG(+AGGACA)GTACCAAAGTGACTTGCTTATGGGGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240613181917_ehn8 ATACTCAAGTCTCCTATTTGTTCAACCAAGGG(+AGGACA)GATCCAATAAGATGTACTAGCGAGTATTTAT NGG 10 8 17 8 24 0 100 5 10
    240613181916_lyqw TGCTCATAAATACTCGCTAGTACATCTTATTG(+AGGACA)GATCCCCTTGGTTGAACAAATAGGAGACTTG NGG 10 8 17 8 24 0 100 5 10
    240613181914_91cj CCAAATATTTATATTTACATACATTTCAAAGG(+AGGACA)GATCCAGTGATTGATATAAGATTCAGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613181912_8p46 CCATGTTAAACTGAATCTTATATCAATCACTG(+AGGACA)GATCCCTTTGAAATGTATGTAAATATAAATA NGG 10 8 17 8 24 0 100 5 10
    240613181911_o3zj AATGCCTGCAAACATTCAGACTAATCAACGTG(+AGGACA)GTACCGTAATTTCTTTTAATACATCAATAAT NGG 10 8 17 8 24 0 100 5 10