Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233852_zt1p TCTTGATGATAATGGAGAGAGGACCCTGAAAG(+ATATAT)GTACCTCCAGTTAGCTTAAGCCAGAGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240606233850_zp89 AAAGAGGCGACTCTGGCTTAAGCTAACTGGAG(+ATATAT)GTACCTTTCAGGGTCCTCTCTCCATTATCAT NGG 10 8 17 8 24 0 100 5 10
    240606233849_1kc2 GGCAGCTCAGCCCTTTGCCTTTTCAGGCTATG(+ATATAT)GATCCCATGTAGCCTGGGAGTTGGCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606233847_oguw TGACTGAGGAGGCCAACTCCCAGGCTACATGG(+ATATAT)GATCCATAGCCTGAAAAGGCAAAGGGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606233845_9ny3 TGTCCTCTGCCCTATCCCTTATCTCAGGAAGG(+ATATAT)GTACCTGTGCAAGATGGGAACTGTCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606233843_5e0w TCAGGGTCTTGGACAGTTCCCATCTTGCACAG(+ATATAT)GTACCCTTCCTGAGATAAGGGATAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606233842_8uaf GGTCAGTATCAAGGAGACATGGGTGGCTACAG(+ATATAT)GATCCCCAAGCTCAGCCCTGGCCCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606233840_of2o CTGGGATCACTTGGGCCAGGGCTGAGCTTGGG(+ATATAT)GATCCTGTAGCCACCCATGTCTCCTTGATAC NGG 10 8 17 8 24 0 100 5 10
    240606233838_qcd3 CTAGGCCAGTGCTACTGTGGACACTGGCTGTG(+ATATAT)GATCCTGGGCTGCTGCCACTCTGCAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606233835_6ljh GTGTATGCTTCTGCAGAGTGGCAGCAGCCCAG(+ATATAT)GATCCACAGCCAGTGTCCACAGTAGCACTGG NGG 10 8 17 8 24 0 100 5 10