Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233947_3kd1 GACAGCAAAGCCATCACAAAGTGGGCTGCGGG(+ATATAT)GATCCAGCCACAGCCACCATGGGCTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606233945_onew ACATGGTGCTCAGCCCATGGTGGCTGTGGCTG(+ATATAT)GATCCCCGCAGCCCACTTTGTGATGGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606233943_zb07 TGTTTCTGGGGCCTCCTTCACGGATTCATATG(+ATATAT)GTACCTTCCTTCTAAACTTGAGGAAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606233942_huc7 AGACAAATGCCTTCCTCAAGTTTAGAAGGAAG(+ATATAT)GTACCATATGAATCCGTGAAGGAGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606233939_hv0b ACTTCTCCCTGTCAGCCCCAGCTGGCCCATGG(+ATATAT)GATCCTGGGACAGCCGACTCATGACCCACCT NGG 10 8 17 8 24 0 100 5 10
    240606233937_axo8 TGTCCAGGTGGGTCATGAGTCGGCTGTCCCAG(+ATATAT)GATCCCATGGGCCAGCTGGGGCTGACAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606233935_1xr6 AGGGCAGCATCTCCTGGGAAAACGAACTAGAG(+ATATAT)GATCCAGAGCCTTGTGGCCCTTTCACGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606233934_oijd TTCTCTGACCGTGAAAGGGCCACAAGGCTCTG(+ATATAT)GATCCTCTAGTTCGTTTTCCCAGGAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240606233932_hb9z GGCTCCCCTGCCTCAGTCTGCAAGGCTACTTG(+ATATAT)GTACCTCCTCACGCTCCGAGAAGTCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240606233931_malc GGTAAGTGTGAGACTTCTCGGAGCGTGAGGAG(+ATATAT)GTACCAAGTAGCCTTGCAGACTGAGGCAGGG NGG 10 8 17 8 24 0 100 5 10