Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234024_mnjs AGCAGGGGGAGGGGTGGTTAGATCTGAAAATG(+ATATAT)GTACCAGTGACTTCTTATCTCTGGGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240606234022_s0hn TCCGGAAGTCACCCAGAGATAAGAAGTCACTG(+ATATAT)GTACCATTTTCAGATCTAACCACCCCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606234019_jh7v ACTTGAATCTCACGTGGCAGGAGGGGCTATGG(+ATATAT)GATCCGCTCGTCTTCCTCACTGCCGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606234018_w0g4 GGGGAGCTGTGCGGCAGTGAGGAAGACGAGCG(+ATATAT)GATCCCATAGCCCCTCCTGCCACGTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240606234017_p687 AATCAACCAATCATATGAAAAAGAACTTTGTG(+ATATAT)GTACCTAGAGGGGCAGAGCCTGCAAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606234015_ziyh CAGGGCCCACCTTGCAGGCTCTGCCCCTCTAG(+ATATAT)GTACCACAAAGTTCTTTTTCATATGATTGGT NGG 10 8 17 8 24 0 100 5 10
    240606234013_et3z CCCCTGGGAAAGAATTTTTTAAAAGGGCTAGG(+ATATAT)GTACCATTCATCCAATATTTATAGAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240606234012_n2cc TTCTGCCATTTTCTATAAATATTGGATGAATG(+ATATAT)GTACCCTAGCCCTTTTAAAAAATTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606234010_iv0v AATACACATGTGCCAAAACTTGTCTGTAGAGG(+ATATAT)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606234008_3r7b CTGCCTACTGCACGCACACGGCCCATGAGTCG(+ATATAT)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10