Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234123_9xef GTTTAAAAAAATACAATTAACATGGGAACAAG(+ATATAT)GATCCCATTTTTTTAAAAAGAAAAAATATAT NGG 10 8 17 8 24 0 100 5 10
    240606234121_ppi1 ATTATATATATTTTTTCTTTTTAAAAAAATGG(+ATATAT)GATCCTTGTTCCCATGTTAATTGTATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606234118_fcpf CTCTAAAAGGCACAGGTATCCTGTATGAATAG(+ATATAT)GATCCCACCCTTTACCTGCAGTCACTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606234116_6i7x TAGGGTTTGGAGTGACTGCAGGTAAAGGGTGG(+ATATAT)GATCCTATTCATACAGGATACCTGTGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606234114_1gwn CGAGGTAGCTGGAGACGCTTACGGAAGGGGTG(+ATATAT)GATCCCATGGGACCTGAGGTCCTCCTCACCC NGG 10 8 17 8 24 0 100 5 10
    240606234112_4dyd GTCAGGGGTGAGGAGGACCTCAGGTCCCATGG(+ATATAT)GATCCACCCCTTCCGTAAGCGTCTCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606234109_t8v7 CCCGTGTTGCCCTCAGGCTGTGGCAGTGGAGG(+ATATAT)GTACCGGGCAGGCATTGCCTAAGGCCCTACT NGG 10 8 17 8 24 0 100 5 10
    240606234106_957z ACAGTAGTAGGGCCTTAGGCAATGCCTGCCCG(+ATATAT)GTACCCTCCACTGCCACAGCCTGAGGGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606234104_38n0 TAATGGTCATCCTCGCTGTAGCTAGGTAGAGG(+ATATAT)GTACCCCACAAATGCGAATGAGTCTGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606234102_o65x CAGGAGACAACAGACTCATTCGCATTTGTGGG(+ATATAT)GTACCCTCTACCTAGCTACAGCGAGGATGAC NGG 10 8 17 8 24 0 100 5 10