Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234240_umfr AGACTGCAGCATTGGAGGCTCAGGGACCTTGG(+ATATAT)GTACCTAAAGACAGACCTTGGGAAGCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606234238_8l6f CTTCCCTTGGGCTTCCCAAGGTCTGTCTTTAG(+ATATAT)GTACCCAAGGTCCCTGAGCCTCCAATGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606234237_edfh AAATCATACCAAATGCACTCCACACTTTTTAG(+ATATAT)GTACCTCCTTCCATCCACAGAAAGTAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606234235_0e9m AACTGTGGCCTACTTTCTGTGGATGGAAGGAG(+ATATAT)GTACCTAAAAAGTGTGGAGTGCATTTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606234233_ybov CTCACCTTCCTGCTGGAGGCTTTGACTGCTGG(+ATATAT)GTACCATGAGAAACACTGAGTATCAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606234232_893b CCTACACTGACTGATACTCAGTGTTTCTCATG(+ATATAT)GTACCCAGCAGTCAAAGCCTCCAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606234230_2hb3 ATAGAGAAATAAATATAGTGTGGAGTTTATGG(+ATATAT)GTACCCCTCAGTCTGCTAATCTTAGTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606234227_lrin GATGTTGAACACTAAGATTAGCAGACTGAGGG(+ATATAT)GTACCCATAAACTCCACACTATATTTATTTC NGG 10 8 17 8 24 0 100 5 10
    240606234226_1z9u GTTTCTGTGTGCTAAGAGTCCAAGTCCACACG(+ATATAT)GTACCTCTGACTCCGTACTTGTTCCATTCAT NGG 10 8 17 8 24 0 100 5 10
    240606234225_edg1 TTAAAATGAATGGAACAAGTACGGAGTCAGAG(+ATATAT)GTACCGTGTGGACTTGGACTCTTAGCACACA NGG 10 8 17 8 24 0 100 5 10