Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234319_xgzk ACAGTAAAAGTGATGTCGCTTGAGAGAAGGTG(+ATATAT)GTACCCTCGGTGCCGGCTTGCACACTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606234316_fltu TACTTCAGCTAGTGTGCAAGCCGGCACCGAGG(+ATATAT)GTACCACCTTCTCTCAAGCGACATCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606234314_h0bt TTGGGGTCCCTCAGCACTTTGCCCTGATTTCG(+ATATAT)GATCCTGAGTAACAGATACCACTGAATTGCT NGG 10 8 17 8 24 0 100 5 10
    240606234313_65h5 GTTACAGCAATTCAGTGGTATCTGTTACTCAG(+ATATAT)GATCCGAAATCAGGGCAAAGTGCTGAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606234310_t75s GCGGCCCCAGCAACCGGGACGGAGGCTGCCGG(+ATATAT)GATCCAGCCCGCGCCGCCAACGTGCGGTCGG NGG 10 8 17 8 24 0 100 5 10
    240606234308_2sgm GGGTTCCGACCGCACGTTGGCGGCGCGGGCTG(+ATATAT)GATCCCGGCAGCCTCCGTCCCGGTTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606234306_abro ATGTTCCTGTATTTCTGCCCCTTGAGTGTTGG(+ATATAT)GATCCCAGGCACAGACCTCACCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606234304_tp44 TCCTCTGGCTGGGTGGTGAGGTCTGTGCCTGG(+ATATAT)GATCCCAACACTCAAGGGGCAGAAATACAGG NGG 10 8 17 8 24 0 100 5 10
    240606234302_t30f AAGGAACCCACTATAATTAAGAGTAGCAAGAG(+ATATAT)GTACCAGTAGGGGCTGTGTAATTCAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606234301_5irf TCTTGTTTTATTGAATTACACAGCCCCTACTG(+ATATAT)GTACCTCTTGCTACTCTTAATTATAGTGGGT NGG 10 8 17 8 24 0 100 5 10