Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234339_9buf GTCTGTTTTACACTAGGTTCTAGAAGTAATTG(+ATATAT)GATCCCAAAGTTCTTTGGCTATTATAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606234337_4xne GTCTATACTTTATAATAGCCAAAGAACTTTGG(+ATATAT)GATCCAATTACTTCTAGAACCTAGTGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606234335_ffth GAAAACGAGACTCTATATGCCTTTTGACCATG(+ATATAT)GATCCTGTGTTTGAATGACAGGCATTAATTT NGG 10 8 17 8 24 0 100 5 10
    240606234333_vau1 AGACCAAATTAATGCCTGTCATTCAAACACAG(+ATATAT)GATCCATGGTCAAAAGGCATATAGAGTCTCG NGG 10 8 17 8 24 0 100 5 10
    240606234331_kt2w TTATAGGCCTTGAAACATAAAAGGCAATCCAG(+ATATAT)GTACCAACACAGCGCACACACGACACAAATG NGG 10 8 17 8 24 0 100 5 10
    240606234330_chx5 TCTTTCATTTGTGTCGTGTGTGCGCTGTGTTG(+ATATAT)GTACCTGGATTGCCTTTTATGTTTCAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606234328_57o4 TGCTGATTTAAGCAGAACACAAAACAAATATG(+ATATAT)GATCCTAGGGCTTACTTCCACCAGTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240606234325_dl2w TCCACGCTGGTACTGGTGGAAGTAAGCCCTAG(+ATATAT)GATCCATATTTGTTTTGTGTTCTGCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606234323_t5md AAACTAGGAAAGATGGGAGGCAGAGACAGGAG(+ATATAT)GATCCCTGGGTTCAAAGGTCTACAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606234320_lrg6 GGGGTTTCTCTTTGTAGACCTTTGAACCCAGG(+ATATAT)GATCCTCCTGTCTCTGCCTCCCATCTTTCCT NGG 10 8 17 8 24 0 100 5 10