Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234447_6qsz GGGCTGCCTGACAGCGTCACAGCTGGGAATGG(+ATATAT)GATCCCAAAATCGTTTCCTGTTTGCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240606234445_8x05 TAGAGGAAGATGCAAACAGGAAACGATTTTGG(+ATATAT)GATCCCATTCCCAGCTGTGACGCTGTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606234443_nd2e CCTCTAGTGACTCCCCACTGACAGATCATTGG(+ATATAT)GATCCATTGTGCATCACGATGGTGATGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606234442_8r6u TTCACCCTCCATCACCATCGTGATGCACAATG(+ATATAT)GATCCCAATGATCTGTCAGTGGGGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240606234440_hjge AAAAGAAAGAAAGAAACTGTTCTTTTCATTTG(+ATATAT)GTACCATAGTGTGAAGAGCTGGGAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240606234439_zs2y AACAGTCATTGCTCCCAGCTCTTCACACTATG(+ATATAT)GTACCAAATGAAAAGAACAGTTTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606234438_qcxr CAAGCTCAGGTTCTCCCATCCAGGGGGAAGTG(+ATATAT)GTACCCACGTTGACTAGAATGGGAAGATGTT NGG 10 8 17 8 24 0 100 5 10
    240606234436_f9lg TTGGGAACATCTTCCCATTCTAGTCAACGTGG(+ATATAT)GTACCACTTCCCCCTGGATGGGAGAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240606234435_e7aj ATGTACTTCATGAGAAAATTCAACAGCAGCTG(+ATATAT)GTACCGGATTCCAAGAAGGAAGGAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240606234433_baok CACCCGTGTGCTTCCTTCCTTCTTGGAATCCG(+ATATAT)GTACCAGCTGCTGTTGAATTTTCTCATGAAG NGG 10 8 17 8 24 0 100 5 10