Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234538_ethm CAGGATAATGATACCATAAGTGTCATGGGAGG(+ATATAT)GATCCTGGTGCACAAAAACTGTTTAAAGATA NGG 10 8 17 8 24 0 100 5 10
    240606234537_zbes GGCCCTATCTTTAAACAGTTTTTGTGCACCAG(+ATATAT)GATCCCTCCCATGACACTTATGGTATCATTA NGG 10 8 17 8 24 0 100 5 10
    240606234535_wvbw TCTTAAGTGAGCACTAACTTGTTACAGTGCTG(+ATATAT)GATCCAGCCAGCCCCAGAGGCCCAATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606234534_cheb AGTTAATGAGATTGGGCCTCTGGGGCTGGCTG(+ATATAT)GATCCAGCACTGTAACAAGTTAGTGCTCACT NGG 10 8 17 8 24 0 100 5 10
    240606234533_50wt AAAAGGACACATAGTATATAACTCCACTGTTG(+ATATAT)GTACCAACAATATTTACTGCAGAAAACCACG NGG 10 8 17 8 24 0 100 5 10
    240606234531_pz0s GTTTCCGTGGTTTTCTGCAGTAAATATTGTTG(+ATATAT)GTACCAACAGTGGAGTTATATACTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606234529_ekq3 AAGTAGAAAATCATCTTTAAAAGACAGCCAAG(+ATATAT)GTACCATCACATCTCCCTGAAAGTAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606234528_gdpq CTGTTCTGTCTTACTTTCAGGGAGATGTGATG(+ATATAT)GTACCTTGGCTGTCTTTTAAAGATGATTTTC NGG 10 8 17 8 24 0 100 5 10
    240606234527_839i ATTGATTCCTACTTTATTATCTTAAAGACTAG(+ATATAT)GATCCTGTTATTTTTCTTTAAATGTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606234525_hujf AGTGCCTGTGAACATTTAAAGAAAAATAACAG(+ATATAT)GATCCTAGTCTTTAAGATAATAAAGTAGGAA NGG 10 8 17 8 24 0 100 5 10