Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234640_nye5 CTGACAGCCACTTGTAATTCATGCTCCAGAGG(+ATATAT)GATCCTATGCCTCTGGCCTCCTCAAGCACCC NGG 10 8 17 8 24 0 100 5 10
    240606234638_1e6q CCAACAATAAAAATAAATCTTAAAGGGGGAGG(+ATATAT)GTACCAACAGTGCCTGACACTACATGAACTC NGG 10 8 17 8 24 0 100 5 10
    240606234636_422o AGGAAGAGTTCATGTAGTGTCAGGCACTGTTG(+ATATAT)GTACCCTCCCCCTTTAAGATTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240606234633_a0nu TTTAGGACTGACACCCTGAGTTTAGTCCCTGG(+ATATAT)GATCCTCGTGGTGGGAGAGAGAACTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240606234632_wqne GTGTGGAAGTCAGTTCTCTCTCCCACCACGAG(+ATATAT)GATCCCAGGGACTAAACTCAGGGTGTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240606234630_eg0r CCTGCTTCGACTTGACTTGCCAGGGCGGGTTG(+ATATAT)GTACCCATGGGGAGGCCTCCCCTTCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606234628_grf2 TTTCCTTCTGAGAAGGGGAGGCCTCCCCATGG(+ATATAT)GTACCAACCCGCCCTGGCAAGTCAAGTCGAA NGG 10 8 17 8 24 0 100 5 10
    240606234627_yc6m CTTTTTGAAAGTAAATACTTTATGTAGCAAAG(+ATATAT)GTACCTGCATAATTGTTGGCACCTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240606234626_r5bb TAGTGTATAAAAAGGTGCCAACAATTATGCAG(+ATATAT)GTACCTTTGCTACATAAAGTATTTACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606234624_yeig CTTTTTGAAAGTAAATACTTTATGTAGCAAAG(+ATATAT)GTACCTGCATAATAGTGGGCACCTTTTTATA NGG 10 8 17 8 24 0 100 5 10