Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234709_zo8m GTTGGTTGTTCTTTGCAAATAGCTGTTACCCG(+ATATAT)GATCCAGCTTGCCCACCTGGCAGGATTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606234707_6ooz GCACTTCTTGCTCTAGCATCTTCTTCAAGTGG(+ATATAT)GATCCAGCAAAGGAGGCTGAGATGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606234706_yxaf CATGACCTTAGACATCTCAGCCTCCTTTGCTG(+ATATAT)GATCCCACTTGAAGAAGATGCTAGAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606234704_e3be AAAACTAGGTTGGAATTGAAACATTTTGTAAG(+ATATAT)GTACCTTAATCCTTGGTAATTATTAGCATAT NGG 10 8 17 8 24 0 100 5 10
    240606234703_e95a TAATCATATGCTAATAATTACCAAGGATTAAG(+ATATAT)GTACCTTACAAAATGTTTCAATTCCAACCTA NGG 10 8 17 8 24 0 100 5 10
    240606234702_gz7w GACATCATCACTGTTCAGCGGTCGGCTTTGTG(+ATATAT)GATCCAGAGAGAGAAGCATGCTACAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606234700_afrd CGCTCACTCTCTGTAGCATGCTTCTCTCTCTG(+ATATAT)GATCCACAAAGCCGACCGCTGAACAGTGATG NGG 10 8 17 8 24 0 100 5 10
    240606234659_4neh CGAGGGACAGGGACGGTGGGCTCCTCGTATGG(+ATATAT)GTACCTTTGAAGCAAGTCCAGTCTTTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606234658_fv8t GTGTTATTTTAAAGACTGGACTTGCTTCAAAG(+ATATAT)GTACCCATACGAGGAGCCCACCGTCCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606234656_am41 ACCATGCCTTGGCCTCATTTTCTTCTGGCCAG(+ATATAT)GTACCATTGTTGGCTATTAAAAAGAACTGTT NGG 10 8 17 8 24 0 100 5 10