Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234609_e4hf AAACTACATTTTTTTCTCTTGACTTCCTGTGG(+ATATAT)GATCCCCATAGCAGTCTTTCAGATAAAGATT NGG 10 8 17 8 24 0 100 5 10
    240606234607_b2r7 ATCTTAATCTTTATCTGAAAGACTGCTATGGG(+ATATAT)GATCCCACAGGAAGTCAAGAGAAAAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606234605_jtib AGAAATTCAGGGCTGAAGAATAGACCCAGGCG(+ATATAT)GATCCAGGCACAGCCAGATCCACCCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606234603_vulo TCTTAGCTACAGGGTGGATCTGGCTGTGCCTG(+ATATAT)GATCCGCCTGGGTCTATTCTTCAGCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606234602_cwly TTGAAGTCCCACTGTTGGCAGTTTTGGCAGTG(+ATATAT)GTACCTAACTCATCGTGTTTGCATTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606234601_b2qb AACTTAACAAGAATGCAAACACGATGAGTTAG(+ATATAT)GTACCACTGCCAAAACTGCCAACAGTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606234559_1vvt AGAAGTCAATGCTGTTCAGATGAAGTCTCTAG(+ATATAT)GTACCAAACCAACATGTTCTCTTTTATCAAT NGG 10 8 17 8 24 0 100 5 10
    240606234558_akw6 CGAGCATTGATAAAAGAGAACATGTTGGTTTG(+ATATAT)GTACCTAGAGACTTCATCTGAACAGCATTGA NGG 10 8 17 8 24 0 100 5 10
    240606234557_fwkq GCAACTGGAGGGGCCGTGAGGAGGAGGAGCTG(+ATATAT)GTACCTTGCTAGGCGGAAGCAGGCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606234555_nznb CTCTGTCACAGTGCCTGCTTCCGCCTAGCAAG(+ATATAT)GTACCAGCTCCTCCTCCTCACGGCCCCTCCA NGG 10 8 17 8 24 0 100 5 10