Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234724_p0i4 CCAAAAGATGTGGTCTCTCAACCTGTCCTGGG(+ATATAT)GTACCTAGTTGTTTATAAATACCTTGGACCA NGG 10 8 17 8 24 0 100 5 10
    240606234722_7x9i AGATGTTGCATGCACCAACTGAGCTATCATTG(+ATATAT)GATCCAAGTGGTCAGCGCTGAAATCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606234721_wr79 ACGGAAGGAGAGATTTCAGCGCTGACCACTTG(+ATATAT)GATCCAATGATAGCTCAGTTGGTGCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240606234719_v6pc CCCTTCCTGAGGACTGTAACTCCAGCTCTGGG(+ATATAT)GATCCAGTGTCCTCTCGTGCCCTCCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606234718_oguq GTGCATGCTCTGGAGGGCACGAGAGGACACTG(+ATATAT)GATCCCCAGAGCTGGAGTTACAGTCCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606234717_sg0m CGCTGCTTGTATATGAAAATAAAAGGCATACG(+ATATAT)GTACCCCTGAGACAAAAGCCGAGGGGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606234715_6evq GAATAACACCCCCCTCGGCTTTTGTCTCAGGG(+ATATAT)GTACCGTATGCCTTTTATTTTCATATACAAG NGG 10 8 17 8 24 0 100 5 10
    240606234714_imfs CTGTGAGGTCAAGGCATCGAGCGGCATAGTAG(+ATATAT)GATCCTGTCTCAAGCACACCGGCTAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606234713_63n1 AGCTACCACTCTAGCCGGTGTGCTTGAGACAG(+ATATAT)GATCCTACTATGCCGCTCGATGCCTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606234711_7vvu CAGGAGACCAATCCTGCCAGGTGGGCAAGCTG(+ATATAT)GATCCGGGTAACAGCTATTTGCAAAGAACAA NGG 10 8 17 8 24 0 100 5 10