Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234740_z4sa GTGGTCCTTGTGGTAACATCAGGACTTTCTTG(+ATATAT)GTACCTTGGTTAAATCTACACAGGGTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240606234738_7wjf TGCAATAGCTACAGACCAAACTGGCACAAACG(+ATATAT)GATCCTGTCCAACTGCATTGGTTTTCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606234737_e1qq TTTGTACCTTGAAAACCAATGCAGTTGGACAG(+ATATAT)GATCCGTTTGTGCCAGTTTGGTCTGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606234735_ta4o GTCCTCCAGGCTCCACCAAGACACACACCGGG(+ATATAT)GTACCACATGGTCCATTTCATGTGATCTATT NGG 10 8 17 8 24 0 100 5 10
    240606234733_z0ga AGAGTAATAGATCACATGAAATGGACCATGTG(+ATATAT)GTACCCCGGTGTGTGTCTTGGTGGAGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606234731_lgw0 ACCTCGTCTTCCCCTCCTTCAGGTTAGCCTGG(+ATATAT)GTACCAGTAGCACGTCTCCTAGTGGCCGGCA NGG 10 8 17 8 24 0 100 5 10
    240606234730_nd2g AAGGGTGCCGGCCACTAGGAGACGTGCTACTG(+ATATAT)GTACCCAGGCTAACCTGAAGGAGGGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606234729_gcvv TCAGCGGAATGGCGGATGTGCTTAACAGTCAG(+ATATAT)GATCCTACTGACAAGTGCTGACCATTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606234728_9me6 ACCCAGTAGAAATGGTCAGCACTTGTCAGTAG(+ATATAT)GATCCTGACTGTTAAGCACATCCGCCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606234726_nc11 GAACATGGTCCAAGGTATTTATAAACAACTAG(+ATATAT)GTACCCCAGGACAGGTTGAGAGACCACATCT NGG 10 8 17 8 24 0 100 5 10