Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234841_e7xk AATTCCAGTCTATTGTGTAACCCACCACCAGG(+ATATAT)GATCCCTTAGTAAACACCCAATTATGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606234840_68ag CGAGTGGTCATGCCCTCCTTTTCATTCATCTG(+ATATAT)GTACCAACACCAGAGGGAGAGCATACAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606234839_veyp GGGAAGTCCTGTATGCTCTCCCTCTGGTGTTG(+ATATAT)GTACCAGATGAATGAAAAGGAGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240606234837_f1wj TGTGGAAGAAGCTTGCTGCCTGAGGGCTGAGG(+ATATAT)GATCCATAGGATCTCAATGAGGCTTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606234835_mepr CATGTAACTGCAAGCCTCATTGAGATCCTATG(+ATATAT)GATCCCTCAGCCCTCAGGCAGCAAGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606234834_x89v CATCAAGCAACTCTGAGTCTCTCTGGCTTCTG(+ATATAT)GATCCTGCTACAATATATGTGGGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606234833_9yt1 CCCTTTGTTCCTGCCCACATATATTGTAGCAG(+ATATAT)GATCCAGAAGCCAGAGAGACTCAGAGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606234831_olz8 ACGGCCAAGCCATCCAAAACTAAGGGAAGTGG(+ATATAT)GATCCCTAAGAGGTGAACCCTTAAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606234830_xond TGGGGACAACACTTAAGGGTTCACCTCTTAGG(+ATATAT)GATCCCACTTCCCTTAGTTTTGGATGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606234828_chpn TTTATATTCCAATTATGCCAACAAAGGGCAAG(+ATATAT)GTACCAGGAAAAGCATTAAACTATATTTTTT NGG 10 8 17 8 24 0 100 5 10