Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234913_sdr4 TCATCACAGCCAGGTGGGTGATGCGGAAGTGG(+ATATAT)GTACCCTGGGAGTTGCTGGTCAGAGCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606234912_efd4 CACCAAGAGCAGAGGTACTGCTGGGATTATAG(+ATATAT)GATCCTACAATTCTCACTGGACCTGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606234910_1yf7 GGGTGCCCTTCCAGGTCCAGTGAGAATTGTAG(+ATATAT)GATCCTATAATCCCAGCAGTACCTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606234909_1qtp ATAAGTAATATGCCATTTCACCAAATACACTG(+ATATAT)GATCCCTCTCACAAACACTACCACACTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606234908_ojws AGTGCATAAAGTGTGGTAGTGTTTGTGAGAGG(+ATATAT)GATCCAGTGTATTTGGTGAAATGGCATATTA NGG 10 8 17 8 24 0 100 5 10
    240606234906_0ql4 GGTGACACACTAAAGGAGAGCCCAGACATTAG(+ATATAT)GATCCAAAAAGCAGTTCAACAAACTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606234905_k4jc CTGAAAAAAAAAGTTTGTTGAACTGCTTTTTG(+ATATAT)GATCCTAATGTCTGGGCTCTCCTTTAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606234903_ckd0 TGCACACTGCTACATCTGTGCGGGGTGCCTAG(+ATATAT)GTACCTTGGCAGGAAAGCAAGAACAGTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606234901_mka2 CACTTCTAGACTGTTCTTGCTTTCCTGCCAAG(+ATATAT)GTACCTAGGCACCCCGCACAGATGTAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606234859_h9tw TAACACAGAAGGGGAAAGACACTGTTGAGAGG(+ATATAT)GATCCCAGAGAGGGAGGGAGAGAACGAAGGA NGG 10 8 17 8 24 0 100 5 10