Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234930_hrg1 CAGACTGATTATTTGAGGAGTGTAGTTAATGG(+ATATAT)GATCCTACTCCAGTGATTTTTGTGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606234929_0afq AGGATATTTTCAGTTTGTAATATAAAATTTAG(+ATATAT)GTACCCAGAACCCTCAAAAGACACAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240606234928_n3sb ATCCATGAGGTTGTGTCTTTTGAGGGTTCTGG(+ATATAT)GTACCTAAATTTTATATTACAAACTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606234926_lik0 ACCACATGGTGACTCACAACCATCTGTAATGG(+ATATAT)GATCCCATGCCCTTTTCTACTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606234924_9rn2 TGTCTTCAGACACACAGTAGAAAAGGGCATGG(+ATATAT)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606234922_l2ld TTACAGAGACCAGGCAGCCATGGACAGTGAGG(+ATATAT)GTACCCAATGTAACCACAAGGCCAGGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606234921_vcup CTGGGACCTGCCTGGCCTTGTGGTTACATTGG(+ATATAT)GTACCCTCACTGTCCATGGCTGCCTGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606234919_wm0x AAAGGACACTTTCCCATTCCCTGTACCACCAG(+ATATAT)GTACCAGATATGAGCTGGATATATGGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606234918_sb57 TGAGGCTTGGCCATATATCCAGCTCATATCTG(+ATATAT)GTACCTGGTGGTACAGGGAATGGGAAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606234914_62ad TGTAAAACAAGCTCTGACCAGCAACTCCCAGG(+ATATAT)GTACCCACTTCCGCATCACCCACCTGGCTGT NGG 10 8 17 8 24 0 100 5 10