Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234947_vn8v TTTTCCTCTGACTGACACTGAAGAGCAAATTG(+ATATAT)GTACCTGGGCTGCTTTCTATTTGTCTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606234945_60sc GGGTGGGTGAGTGCTGGCATGGACGTCTCTAG(+ATATAT)GATCCTCTACAAACAGGCCCAGTTCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240606234944_tm3d TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+ATATAT)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240606234942_6iqw GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+ATATAT)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606234940_y233 AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+ATATAT)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10
    240606234938_0iyi TGTTTAGACACATTTTACTAGGCACCTCCGTG(+ATATAT)GATCCTTTACAGTGTTCTTGTGACAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240606234937_vq3j CCACACTAGCTTGTCACAAGAACACTGTAAAG(+ATATAT)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606234935_hyf1 GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+ATATAT)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606234933_2zwb GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+ATATAT)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606234932_miux ACACAACTCCCCCACAAAAATCACTGGAGTAG(+ATATAT)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10