Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235057_hiwb CAACACTTAACATGATACAAGAGTTAGAAATG(+ATATAT)GATCCGCTCCTTGTCACTCCCAAGAGATGCG NGG 10 8 17 8 24 0 100 5 10
    240606235056_07pj TCATGGCTATTGAGAGGGTCTGAGGGCTGTGG(+ATATAT)GTACCCTGGCCACACTCCTGGGGGCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606235053_solw GCTACCACAGAGCCCCCAGGAGTGTGGCCAGG(+ATATAT)GTACCCACAGCCCTCAGACCCTCTCAATAGC NGG 10 8 17 8 24 0 100 5 10
    240606235051_oedk TTGGGAAGGAGGGCTGTGGTGTGGACCACCTG(+ATATAT)GATCCCTATAGGAGAGACCCTGACTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606235049_5plg CCACTGAGCACAGTCAGGGTCTCTCCTATAGG(+ATATAT)GATCCAGGTGGTCCACACCACAGCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606235045_dsxf AGATTGGCATCAGAGGCCCCGACTCCCCCCGG(+ATATAT)GATCCGGGAGCCTAGCCCATCCCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606235043_tzrx GCGCATGCTGCCAGGGATGGGCTAGGCTCCCG(+ATATAT)GATCCCGGGGGGAGTCGGGGCCTCTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240606235041_3kn1 ACATCTGCTCCTGGGGATATACGGGAGAGGAG(+ATATAT)GTACCATAGCATCAAGTTTGGGGAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240606235040_5wq6 GCACCACAGTCCTCCCCAAACTTGATGCTATG(+ATATAT)GTACCTCCTCTCCCGTATATCCCCAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606235039_vfxm GCCTGGCAGTGCTGGGAAAGTCTCTGTAAGCG(+ATATAT)GATCCAGAGTGTTCGAGGCACAGCCCTGGGT NGG 10 8 17 8 24 0 100 5 10