Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235112_65wz CACACGCTAGGCAGTATTTACTGATACACCGG(+ATATAT)GTACCAAACACCTCTCAGACTCTGGAATAGA NGG 10 8 17 8 24 0 100 5 10
    240606235109_gf23 AGTGAAGTAAAAGATTAGGGTAAGCTTCCCGG(+ATATAT)GATCCAGATAAAAGATTATTTCTGTATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606235108_zd43 GCTAAGACTATACAGAAATAATCTTTTATCTG(+ATATAT)GATCCCGGGAAGCTTACCCTAATCTTTTACT NGG 10 8 17 8 24 0 100 5 10
    240606235106_g0w0 AGTCAAGGCCTGGGCAGTGTCTAGTGGAGAGG(+ATATAT)GTACCAAGACCAGCAGAGAGAAGCCACCTAA NGG 10 8 17 8 24 0 100 5 10
    240606235105_ppuv GCCACTTAGGTGGCTTCTCTCTGCTGGTCTTG(+ATATAT)GTACCCTCTCCACTAGACACTGCCCAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606235103_7dzy ACAAGCACTTGTCATACATTGATACTGGATTG(+ATATAT)GTACCATTCTAGAGCACAAGGCCTAAATCAT NGG 10 8 17 8 24 0 100 5 10
    240606235102_c7l2 TTTTAATGATTTAGGCCTTGTGCTCTAGAATG(+ATATAT)GTACCAATCCAGTATCAATGTATGACAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606235101_3jwr TGGCCTCAGACCTGATCAAGACAATATCGGAG(+ATATAT)GATCCAAAGCCATTCTCTGGTTCGCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606235100_zk0y TAAATAAAGAAGCGAACCAGAGAATGGCTTTG(+ATATAT)GATCCTCCGATATTGTCTTGATCAGGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606235059_up9n GAGAGCGCATCTCTTGGGAGTGACAAGGAGCG(+ATATAT)GATCCATTTCTAACTCTTGTATCATGTTAAG NGG 10 8 17 8 24 0 100 5 10