Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235201_g2nj GTAGAGTTCGCCTAGCATGCATACGAAGCCTG(+ATATAT)GATCCCATATCCCAGCAGGGCAAACCAATTG NGG 10 8 17 8 24 0 100 5 10
    240606235200_xqt9 GGGCAGCCACCCCATATCCCATGGGGCGGGAG(+ATATAT)GATCCAGTTAGGAGCTGAAAACCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606235158_txy9 TCTTGTCCAGCAGGGTTTTCAGCTCCTAACTG(+ATATAT)GATCCTCCCGCCCCATGGGATATGGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606235157_mj9u CTACAAACCTTGACTAATGGCACAGTGCACAG(+ATATAT)GTACCAAGTGCCCCACCCCACCCCAGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606235155_mceg TGATTGGGGACTGGGGTGGGGTGGGGCACTTG(+ATATAT)GTACCTGTGCACTGTGCCATTAGTCAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606235154_rff6 GGTTGAAATTCAAGATAAGAGCTCAGAGCTTG(+ATATAT)GATCCAGACAGGAGCAGGGGCAGGTGGATGT NGG 10 8 17 8 24 0 100 5 10
    240606235153_wynm CACCAACATCCACCTGCCCCTGCTCCTGTCTG(+ATATAT)GATCCAAGCTCTGAGCTCTTATCTTGAATTT NGG 10 8 17 8 24 0 100 5 10
    240606235151_wjnk GACATTGTGCTCATCTCGATGTCAGCAAAAGG(+ATATAT)GATCCACTCCTTCCTATGAGCAAACCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606235150_ebiw AAACAGACCAGGTTTGCTCATAGGAAGGAGTG(+ATATAT)GATCCCTTTTGCTGACATCGAGATGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606235148_v645 CTTTGCTGCTGCAAGGAGAATGTCTCTCTTGG(+ATATAT)GTACCAGTGGGTCCTTCACACCTTCCCACAA NGG 10 8 17 8 24 0 100 5 10