Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234432_zbg9 AAGTCACAGACTTTAAAAGTAGGTATGGGCGG(+ATATAT)GATCCATCCAAGCCATCACTGGTCAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606234430_h7o8 GATGTCTCTCCTGACCAGTGATGGCTTGGATG(+ATATAT)GATCCCGCCCATACCTACTTTTAAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606234428_frf4 GTCCCTGTGCTGATGGGGAGGAGGAGGGCAGG(+ATATAT)GTACCGGGAGCAGGTAGCAGAAGGAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606234426_adc8 AAGGCAGAGGCTCCTTCTGCTACCTGCTCCCG(+ATATAT)GTACCCTGCCCTCCTCCTCCCCATCAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606234424_3230 GAGCTGACCTTCCCAGGAACCTCACAGTTCCG(+ATATAT)GATCCTTTGTCTCAGTAGATACAGCTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240606234423_29is CATGAGGCTAAGCTGTATCTACTGAGACAAAG(+ATATAT)GATCCGGAACTGTGAGGTTCCTGGGAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606234421_utvo CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+ATATAT)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240606234419_cxyv ATCTAAATTCATAAATGGTATGAGGCACTTGG(+ATATAT)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606234417_7oko CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+ATATAT)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240606234415_37wz ATCTAAATTCATAAATGGTATGAGGCACTTGG(+ATATAT)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10