Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234356_fze1 AAATAAAAAAGAGAAACTCTGGGAACCTCTGG(+ATATAT)GATCCGAGGCAGCCAATACAGTGGTGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606234354_rt4h ATCCAACTTACACCACTGTATTGGCTGCCTCG(+ATATAT)GATCCCAGAGGTTCCCAGAGTTTCTCTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606234352_cri1 TCCACTGTCTAAGTCTGTAACATAACAAACGG(+ATATAT)GATCCTGTCTTCTTCTTTTCTAATAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240606234351_f26g TTACAAAATTCTATTAGAAAAGAAGAAGACAG(+ATATAT)GATCCCGTTTGTTATGTTACAGACTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240606234348_m9ub ACGGGCTAATCTGCTTGTCATGAAGCCCCAAG(+ATATAT)GATCCCTCTCTGCTTCTCCACTGCTGACATT NGG 10 8 17 8 24 0 100 5 10
    240606234346_ug0i GTTGCAATGTCAGCAGTGGAGAAGCAGAGAGG(+ATATAT)GATCCTTGGGGCTTCATGACAAGCAGATTAG NGG 10 8 17 8 24 0 100 5 10
    240606234345_nkz9 TAAAAAGTCTTGCCTCTGAAGACCTTTTAAAG(+ATATAT)GTACCATGAGTAGGAATTGGGAAGAATTGTT NGG 10 8 17 8 24 0 100 5 10
    240606234344_6y9k ATTGTAACAATTCTTCCCAATTCCTACTCATG(+ATATAT)GTACCTTTAAAAGGTCTTCAGAGGCAAGACT NGG 10 8 17 8 24 0 100 5 10
    240606234342_a46u TTTCTTAATGTTCTAATTATAATGAAAGTCGG(+ATATAT)GATCCCATGTCACACTTTGGGAAACCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606234340_1l5m CTAAATCAAAGGTTTCCCAAAGTGTGACATGG(+ATATAT)GATCCCGACTTTCATTATAATTAGAACATTA NGG 10 8 17 8 24 0 100 5 10