Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234259_8uxm GGAGATCTAGTGTACTCTTCTGACCTCCAAAG(+ATATAT)GTACCAGGTGAACATCCGGATGAATGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606234256_3q8h ATTTTGCCTGCATTCATCCGGATGTTCACCTG(+ATATAT)GTACCTTTGGAGGTCAGAAGAGTACACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606234255_whjl TCTAAAATGTATCATACATTAACGCAGGGCAG(+ATATAT)GATCCATTAACATGGCTCCGCCTTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240606234253_97um CTGCCACTTGTAAAGGCGGAGCCATGTTAATG(+ATATAT)GATCCTGCCCTGCGTTAATGTATGATACATT NGG 10 8 17 8 24 0 100 5 10
    240606234251_2wr1 ACGAGTCATTGCTTCTGGTTTGTCCTAACAGG(+ATATAT)GTACCTGACAGGAAATTGCTAGCTCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606234250_zvqj AGTTTCTGCCAGAGCTAGCAATTTCCTGTCAG(+ATATAT)GTACCCTGTTAGGACAAACCAGAAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240606234247_oofo GTAGGGTGTTCCTCCCAGTTCAGGTAGTGTTG(+ATATAT)GATCCTGAGTTGTGGCTGGGATAACTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606234245_n63b AATCCTGAAGAGTTATCCCAGCCACAACTCAG(+ATATAT)GATCCAACACTACCTGAACTGGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606234244_2rlp TTACTCTCAAGGCTTCATGATCGACAATGAAG(+ATATAT)GATCCTGCAGGAACGGAGGTGGTGGTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606234242_ahd9 CATCTGTGCCACCACCACCTCCGTTCCTGCAG(+ATATAT)GATCCTTCATTGTCGATCATGAAGCCTTGAG NGG 10 8 17 8 24 0 100 5 10