Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606234203_fup8 ACATCTGGGGTCAAGAGAAAGGGGAGTGAGAG(+ATATAT)GATCCTCAAAGGATATCTCCTTTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606234201_h668 AGGAAGGAAGGGCAAAGGAGATATCCTTTGAG(+ATATAT)GATCCTCTCACTCCCCTTTCTCTTGACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606234159_ovj6 AGTGTCAAGGTTTGGTGGCTGATTATGGGATG(+ATATAT)GATCCCTGGGAGGGATATTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606234156_zgvy GGATGGACTATCCAGAGAATATCCCTCCCAGG(+ATATAT)GATCCATCCCATAATCAGCCACCAAACCTTG NGG 10 8 17 8 24 0 100 5 10
    240606234155_308t AGGTTCTCCTCTTCTCTGCCAGGGAAAGACAG(+ATATAT)GATCCACACTGCTCTCCCAAACCCTTCCATT NGG 10 8 17 8 24 0 100 5 10
    240606234153_g5ny GCCCCAATGGAAGGGTTTGGGAGAGCAGTGTG(+ATATAT)GATCCTGTCTTTCCCTGGCAGAGAAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606234151_bxlv ACCATTTGCTACCACTCATAGTAATGATAATG(+ATATAT)GATCCGGGTAAGACTCATTGATGGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606234149_bpxj TTCCCCCAGCCTCCATCAATGAGTCTTACCCG(+ATATAT)GATCCATTATCATTACTATGAGTGGTAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606234146_ypza GAAATGATAATAAGATCTCCAAGAACATCTGG(+ATATAT)GATCCAGAAATGACAATAGGAGAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240606234145_3kxj TGATTTCATGAGTTCTCCTATTGTCATTTCTG(+ATATAT)GATCCCAGATGTTCTTGGAGATCTTATTATC NGG 10 8 17 8 24 0 100 5 10