Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233359_64tv TGAGCACTCTCTGGAGGGCTGTTACGCTAATG(+ATATAT)GTACCCCTGTGTCATAATAATCTCTTGACCA NGG 10 8 17 8 24 0 100 5 10
    240606233357_mt9t GGCACATGGGTCAGAACAAAGTTGCATTATGG(+ATATAT)GTACCAGCGATCCAATTAATTAATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240606233355_iv55 CCAGCTATAAATATTAATTAATTGGATCGCTG(+ATATAT)GTACCCATAATGCAACTTTGTTCTGACCCAT NGG 10 8 17 8 24 0 100 5 10
    240606233354_u7wv GTCATGGGCACTGTGGCTGAAGTGGCTGCTTG(+ATATAT)GTACCAGCCGTCTCAGTAGCAGGAGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606233352_arb3 CTTGTGGCTCACTCCTGCTACTGAGACGGCTG(+ATATAT)GTACCAAGCAGCCACTTCAGCCACAGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606233350_8n1p AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+ATATAT)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240606233348_nq4s ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+ATATAT)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606233347_0ly1 CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+ATATAT)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606233345_201q TTACAGTGGCTGTCACACACTCTGAGGACAGG(+ATATAT)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10
    240606233343_njl4 GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+ATATAT)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10