Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230630_v7rx TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606230628_gtvy TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606230626_i2o9 GGGACACAGAAACAGGGGAGCAGATGGGACAG(+ATATAT)GATCCTTTCAGCACCACCTATACCTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606230624_wljq GCACAACCTCCAGGTATAGGTGGTGCTGAAAG(+ATATAT)GATCCTGTCCCATCTGCTCCCCTGTTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606230622_lgvk TGTGTGTACCAGCTGCTTGCACAGGTGTTCTG(+ATATAT)GATCCTGGAGGGCTTCGGGCTCCAGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606230619_5mp9 GGGCTGGCTGGCTGGAGCCCGAAGCCCTCCAG(+ATATAT)GATCCAGAACACCTGTGCAAGCAGCTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240606230617_9fwu AGGCCACTGTAAATTAAGGACTTTCTGAGAAG(+ATATAT)GTACCCACACCTCCTTCCATGCTGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606230615_tah0 ATTAACCTGACCCAGCATGGAAGGAGGTGTGG(+ATATAT)GTACCTTCTCAGAAAGTCCTTAATTTACAGT NGG 10 8 17 8 24 0 100 5 10
    240606230613_3vlc CCTCTGCTTCGGGTACGTACCCATTTCTGTGG(+ATATAT)GTACCTATTAATATCTACTCTGGTATATGCT NGG 10 8 17 8 24 0 100 5 10
    240606230611_ptlx AACACAGCATATACCAGAGTAGATATTAATAG(+ATATAT)GTACCCACAGAAATGGGTACGTACCCGAAGC NGG 10 8 17 8 24 0 100 5 10